The cortical peptidoglycan from spores of Bacillus megaterium and Bacillus subtilis is not highly cross-linked.
نویسندگان
چکیده
Determination by amino acid analyses of the percentage of diaminopimelic acid in the spore cortex of Bacillus megaterium and Bacillus subtilis which is involved in interpeptide cross-links gave values of 31 to 37%. This finding supports the idea that the cortex volume could undergo significant changes in response to changes in pH or ionic strength and could thus play an active role in reducing the water content of the spore protoplast during sporulation.
منابع مشابه
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
Germination of Bacillus spores requires degradation of a modified layer of peptidoglycan (PG) termed the spore cortex by two redundant cortex-lytic enzymes (CLEs), CwlJ and SleB, plus SleB's partner protein, YpeB. In this study, in vitro and in vivo analyses have been used to clarify the roles of individual SleB and YpeB domains in PG degradation. Purified mature Bacillus cereus SleB without it...
متن کاملPurification and properties of a germination-specific cortex-lytic enzyme from spores of Bacillus megaterium KM.
Two peptidoglycan-lytic enzyme activities were isolated from spores of Bacillus megaterium KM. Surface-bound lytic enzyme was extracted from dormant spores and hydrolysed a variety of peptidoglycan substrates including isolated spore cortex, but did not cause refractility changes in permeabilized spores. Germination-specific lytic enzyme activity appeared early in germination and had minimal ac...
متن کاملThe nutritional requirements for germination and outgrowth of spores and vegetative cell growth of some aerobic spore forming bacteria.
During the last 10 years the subject of bacterial spore germination has been intensively investigated. In most of these studies it has been found that bacterial spores are capable of germination under conditions which do not permit the germinated form to survive (Schmidt, 1955; Stedman, 1956). Hills (1949) pointed out that a distinction must be drawn between the requirements for germination and...
متن کاملStructural and functional analysis of SleL, a peptidoglycan lysin involved in germination of Bacillus spores.
A major event in the germination of Bacillus spores concerns hydrolysis of the cortical peptidoglycan that surrounds the spore protoplast, the integrity of which is essential for maintenance of dormancy. Cortex degradation is initiated in all species of Bacillus spores by the combined activity of two semi-redundant cortex-lytic enzymes, SleB and CwlJ. A third enzyme, SleL, which has N-acetylglu...
متن کاملThe Exosporium of Bacillus megaterium QM B1551 Is Permeable to the Red Fluorescence Protein of the Coral Discosoma sp.
Bacterial spores spontaneously interact and tightly bind heterologous proteins. A variety of antigens and enzymes have been efficiently displayed on spores of Bacillus subtilis, the model system for spore formers. Adsorption on B. subtilis spores has then been proposed as a non-recombinant approach for the development of mucosal vaccine/drug delivery vehicles, biocatalysts, bioremediation, and ...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 175 9 شماره
صفحات -
تاریخ انتشار 1993